Chemistry of amino acid derivatization

To make the amino acids fluorescent, a self-made pure fluorescent compound, 6-aminoquinolyl-N-hydroxysuccinimidylcarbamate (AQC), is used for pre-column derivatization. Prepared samples are immediately loaded on ultra-pressure reverse phase chromatography (UPLC). The desired amino acids are separated and detected by means of multi-channel fluorescence detector. The analysis is completed within 6 minutes and is evaluated against internal standards. The quality and reliability of proven amino acids is checked with individual standards of defined concentration.

 

                                                        Copy right: Hajirezaei

Chemical structure of 6-aminoquinolyl-N-hydroxy-succinimidylcarbamate (AQC)     

                Copy right: Hajirezaei

Both primary and secondary amino acids and amines react spontaneously with AQC to produce highly stable, fluorescent derivatives. The excess reagent reacts with water to form free amines.